Helmut Grubmüller and Gunnar F. Schröder


Single molecule Fluorescence Resonance Energy Transfer (FRET) experiments are a powerful and versatile tool for studying conformational motions of single biomolecules at a millisecond time scale. Typically, the small number of recorded photons limits the achieved time resolution. 
  • reconstructs nanometer distance trajectories from time-resolved single photon FRET recordings,
  • extracts the full information from the recorded photon arrival times,
  • provides rigorous error bounds and 
  • is able to extract an effective diffusion coefficient for the distance fluctuations.
FretTrace is the implementation of a maximum likelihood based theory [1], which is described here  in more detail.

 Download  and Installation

 Command line options, Parameters, File Formats